Equipment and Materials

A.

1. Balance, at least 250 g capacity
2. Stirrer or rotating incubator shaker
3. Water bath, 37 ± 0.5°C
4. Beakers, 100 and 1500 ml
5. Sedimentation cone and support, 1 L, plastic with removable plug, e.g., Imhoff cone
6. Tubing, amber gum, 2.4 and 9.5 mm diameter
7. Tubing clamp
8. Microscopes, dissecting and inverted
9. Culture dishes, plastic, various sizes
10. Sieve No. 18 (U.S. standard sieve series), 1 mm mesh, 204 mm diameter, 51 mm high; other sizes optional
11. Tray, rectangular, polypropylene, about 325 × 260 × 75 mm
12. Cylinder, graduated, 1 L
13. pH meter
14. Pasteur pipets, or polypropylene eyedroppers
15. Rubber bulb, about 2 ml capacity
16. Spoon
17. Spatula

Optional Materials

• Blender
• Meat grinder
• Food processor
• Negative pressure hood
• Foil
• Plastic wrap
• Tweezers
• Dissecting needles

Reagents

B.

1. Physiological saline (R63)
2. Pepsin, laboratory grade
3. pH reference solutions
4. HCl, concentrated
5. Optional reagents: papain, ethanol, glacial acetic acid, glycerine, lactophenol, phenol, formalin, Lugol's iodine (R40), ether